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KMID : 0613820090190030384
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Journal of Life Science
2009 Volume.19 No. 3 p.384 ~ p.389
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Relation of Expression Levels of Melanin Synthesis Genes according to the MC1R Genotypes with the Coat Color Patterns in Hanwoo, Jeju Black Cattle and Holstein
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Lee Sung-Soo
Yang Young-Hoon
Cho In-Cheol
Kim Nam-Young
Ko Moon-Suck
Jeong Ha-Yeon
Han Sang-Hyun
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Abstract
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This study was carried out to elucidate the relation between expression levels of three melanin synthesis genes (Tyrosinase, Tyrosinase-related protein 1 and Dopachrome tautomerase) according to the Melanocortin-1 receptor genotypes with coat color patterns in Hanwoo cattle, Jeju black cattle and Holsteins. Using real-time semiquantitative reverse transcription-PCR assay (RT-PCR), the expression levels of these three genes were analyzed in skin tissues from four representative coat colored areas: yellowish-brown from MC1R e/e Hanwoo, wild type black from E?/E? Jeju black cattle (JBC), and dominant black and white pied regions from ED/ED Holstein. The TYR, TYRP1 and DCT genes showed higher expression levels of 4.5, 2.3 and 2.5 times higher in the black skin area of Holsteins than those of from JBC, respectively (p＜0.001). In addition, the expression levels of these three genes from JBC were significantly higher than those from Hanwoo cattle (p＜0.001). These results show that coat color phenotypes in Hanwoo cattle, JBC and Holsteins is directly correlated with TRY, TYRP1 and DCT transcription levels, which probably reflected involvement with MC1R genotypes; e/e in Hanwoo, E?/E? in JBC and ED/ED in Holsteins. Consequently, this study suggested that the status of MC1R protein may not only induce the transcription activities of a series of TYR and its related genes responsible for melanin synthesis, but also determine the levels of total melanin contents in bovine skin.
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KEYWORD
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Melanin, TYR, MC1R, coat color, cattle
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